Espressione proteica si riferisce alla sintesi di proteine within cells using information contained in the DNA or gene.
Proteine are responsible for all the biochemical reactions that take place within the cell. Hence, it makes it necessary to study le proteine function in order to understand cellular processes.
This is currently studied based on using fluorescent proteine as tags. However, this does not provide real time analysis as it requires maturation of chromophore that takes time and this causes a delay in investigation of real-time expression, especially for proteine che sono di natura transitoria o effimera.
I ricercatori hanno segnalato una nuova tecnica sul server di prestampa il 30 luglio 2020 che può superare questa limitazione.
The new study describes the use a fluorescent biosensor that enables real-time detection of le proteine expression in vivo which has implications for understanding the spatiotemporal expression of proteine within the living organism. This sensor is based on a dim green fluorescent le proteine in which pre-existing fluorescence increases 11-fold in vivo following the specific and rapid binding of a le proteine tag and enables detection of le proteine expression within seconds in live cells.
La sezione biosensore will be useful to study biological processes in real-time where proteine are either transiently expressed and/or to understand the expression and interactions of proteine from a disease-causing organism such as bacteria or a virus with that of the host proteins.
Riferimento:
Eason MG., Pandelieva AT., Mayer MM., et al 2020. Biosensore fluorescente geneticamente codificato per il rilevamento rapido dell'espressione proteica. Prestampa: bioRxiv 2020.07.30.229633; DOI: https://doi.org/10.1101/2020.07.30.229633
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